The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 22, 2020

Filed:

Jul. 28, 2016
Applicant:

Arcis Biotechnology Holdings Limited, Warrington, GB;

Inventors:

Jan Rogers, Chester, GB;

Paul Reeves, Liverpool, GB;

Carlos Toro Rueda, Madrid, ES;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6851 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6851 (2013.01); C12Q 2521/107 (2013.01); C12Q 2527/125 (2013.01); C12Q 2531/113 (2013.01);
Abstract

A method of determining of the presence or absence of a target RNA component in a sample, the method comprising steps of: (a) contacting the sample with a composition comprising (i) a quaternary ammonium compound or a precursor thereof, which should extract/release the nucleic acid from the sample; (b) optionally storing and/or transporting the composition obtained in step (a); (c) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent, e.g. BSA or casein for stabilizing the RNA for transport and/or storage; and (d) using the composition obtained in step (c) in an amplification method, e.g. RT-PCR.


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