The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 22, 2020
Filed:
Aug. 22, 2018
Tohoku University, Sendai, JP;
Meiji Seika Pharma Co., Ltd., Tokyo, JP;
National University Corporation Tokyo University of Agriculture and Technology, Fuchu, JP;
Masafumi Goto, Sendai, JP;
Kazutaka Murayama, Sendai, JP;
Youhei Yamagata, Fuchu, JP;
Kimiko Watanabe, Sendai, JP;
TOHOKU UNIVERSITY, Miyagi, JP;
MEUI SEIKA PHARMA CO., LTD., Tokyo, JP;
NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, Tokyo, JP;
Abstract
Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived fromsp. According to this method, the type and amount of protein-degrading enzyme to be used for isolating cells can be determined from the composition of a major protein of the biological tissue. Thus, cells having a high biological activity can be efficiently separated while reducing the amount of protein-degrading enzyme to be used.