The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2020
Filed:
Nov. 04, 2016
California Institute of Technology, Pasadena, CA (US);
Rustem F. Ismagilov, Altadena, CA (US);
Jesus Rodriguez Manzano, London, GB;
Mikhail Karymov, San Jose, CA;
David A. Selck, Sterling, VA (US);
Stefano Begolo, Inglewood, CA (US);
Erik B. Jue, Moorpark, CA (US);
Dmitriy V. Zhukov, Pasadena, CA (US);
California Institute of Technology, Pasadena, CA (US);
Abstract
The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.