The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 15, 2020

Filed:

Feb. 06, 2015
Applicants:

Agency for Science, Technology and Research, Singapore, SG;

National University of Singapore, Singapore, SG;

Inventors:

Filip Laco, Singapore, SG;

Joo Leng Low, Singapore, SG;

Steve Oh, Singapore, SG;

Christina Li Lin Chai, Singapore, SG;

Qixing Zhong, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C07D 401/04 (2006.01); C07D 405/04 (2006.01); A61K 31/4439 (2006.01); A61K 31/506 (2006.01); C07D 413/04 (2006.01); A61K 31/4178 (2006.01); C07D 405/14 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); A61K 31/4178 (2013.01); A61K 31/4439 (2013.01); A61K 31/506 (2013.01); C07D 401/04 (2013.01); C07D 405/04 (2013.01); C07D 405/14 (2013.01); C07D 413/04 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract

This invention relates to a method for inducing or enhancing the differentiation of pluripotent stem cells into cardiomyocyte via casein kinase 1 inhibition said method comprising culturing the stem cells in the presence of a medium comprising a casein kinase 1 inhibitor of the formula (I) or (II) or a stereoisomer, tautomer, or a salt thereof wherein R, Rand Rindependently from another represent hydrogen, optionally substituted alkyl, alkenyl, alkynyl, heterocyclyl, heteroaryl or aryl; X represents NR, O or S; and Rrepresents hydrogen, optionally substituted alkyl, alkenyl, alkynyl, heterocyclyl, heteroaryl or aryl. The method can be used in the late phase of stem cell differentiation and in the compounds of formula (I) or (II) in combination with other small molecules can lead to especially high differentiation of stem cells into cardiomyocytes. The invention further relates to novel compounds which can be used in the method of the invention and kits for stem cell differentiation.


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