The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2020
Filed:
Dec. 07, 2017
Henkel Ag & Co. Kgaa, Duesseldorf, DE;
Patricia Klaka, Leverkusen, DE;
Bernhard Banowski, Duesseldorf, DE;
Sabine Gruedl, Erkelenz, DE;
Thomas Welss, Duesseldorf, DE;
Henkel AG & Co. KGaA, Duesseldorf, DE;
Abstract
The present disclosure relates to a method for improving the culture conditions of cultures of primary sweat gland cells and/or three-dimensional sweat gland equivalents, where the culture with from about 500 to about 500,000 sweat gland cells and a diameter of from about 100 to about 6,000 nm is initially prepared and then cultivated in a culture medium with neurotransmitters and/or from about 11 to about 100 ng/ml of at least one growth factor. Cultivation of these cells and models in a culture medium containing at least one neurotransmitter and/or from about 11 to about 100 ng/ml of at least one growth factor leads to an increase of sweat-gland-specific marker proteins. As a result, a loss of function, which occurs with an absence of or only low expression of these markers, can be avoided.