The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2020
Filed:
Feb. 14, 2018
Grape King Bio Ltd., Zhong-Li, TW;
Pei-Cheng Lin, Zhong-Li, TW;
Han-Hsin Chang, Zhong-Li, TW;
Chin-Chu Chen, Zhong-Li, TW;
Shu-Hsing Yeh, Zhong-Li, TW;
Li-Ya Lee, Zhong-Li, TW;
Jui-Hsia Hsu, Zhong-Li, TW;
GRAPE KING BIO LTD., Zhong-Li, TW;
Abstract
A method of preparingmycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a) culturing amycelium in a plate media at 15 to 35° C. for 5 to 14 days; (b) inoculating the mycelium of step (a) to a flask containing liquid media and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days; (c) inoculating the mycelium of step (b) to a fermenter tank and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days, so as to obtain amycelium fermentation liquid; (d) freeze-drying and grating the fermentation liquid, so as to obtain amycelium powder; (e) extracting the powder with solvent, so as to obtain amycelium extract; and (f) drying the extract, so as to obtained themycelium active substances.