The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2020
Filed:
Dec. 25, 2015
Applicant:
Riken, Saitama, JP;
Inventors:
Hiroki Ueda, Saitama, JP;
Genshiro Sunagawa, Saitama, JP;
Kenta Sumiyama, Saitama, JP;
Maki Ukai, Saitama, JP;
Dimitri Perrin, Saitama, JP;
Assignee:
RIKEN, Saitama, JP;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12N 15/873 (2010.01); C12N 15/11 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0276 (2013.01); A01K 67/027 (2013.01); C12N 15/11 (2013.01); C12N 15/87 (2013.01); C12N 15/873 (2013.01); A01K 2207/05 (2013.01); A01K 2217/075 (2013.01); C12N 2310/20 (2017.05);
Abstract
A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.