The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 08, 2020

Filed:

Apr. 09, 2018
Applicant:

Toppan Printing Co., Ltd., Taito-ku, JP;

Inventors:

Hiroki Nagase, Taito-ku, JP;

Kei Tsukamoto, Taito-ku, JP;

Shiro Kitano, Taito-ku, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C08G 69/10 (2006.01); C08G 73/06 (2006.01); G01N 1/40 (2006.01); C12N 15/09 (2006.01); C07H 1/06 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1006 (2013.01); C07H 1/06 (2013.01); C07H 21/00 (2013.01); C08G 69/10 (2013.01); C08G 73/0611 (2013.01); C08G 73/0616 (2013.01); C12N 15/09 (2013.01); G01N 1/405 (2013.01);
Abstract

A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.


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