The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 08, 2020
Filed:
Apr. 28, 2017
Applicant:
Jcr Pharmaceuticals Co., Ltd., Ashiya, JP;
Inventors:
Assignee:
JCR PHARMACEUTICALS CO., LTD., Ashiya, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 9/16 (2006.01); C12N 15/09 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 9/16 (2013.01); C12Y 301/21001 (2013.01); C12N 15/09 (2013.01);
Abstract
Disclosed is a method for production of recombinant human DNase I which is of such high purity as may be directly used as a medical drug. The method includes the steps of; culturing recombinant DNase I-producing mammalian cells, subjecting a culture supernatant to an anion-exchange column chromatography, subjecting to a column chromatography employing as solid phase a material having affinity for phosphate group, subjecting to a cation-exchange column chromatography, and subjecting to a dye affinity column chromatography.