The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 24, 2020
Filed:
Feb. 01, 2019
Applied Research Associates, Inc., Albuquerque, NM (US);
Alyssa C. Henry, Arlington, VA (US);
Vanessa M. Thomas, Albuquerque, NM (US);
Applied Research Associates, Inc., Albuquerque, NM (US);
Abstract
A cell extraction method includes providing a mixed-cell sample having both target cells and non-target cells slurried in a trailing-electrolyte solution. A leading electrolyte solution is provided to contact the trailing electrolyte solution via a capillary and a pressure control device. Focusing of the target cells at an interface between the leading- and trailing-electrolyte solutions occurs by applying pressure to the capillary and concurrently applying an electric potential via electrodes. The target cells are extracted into the capillary by applying a negative pressure and transferred to a receptacle. A cell extraction system for use with a gradient elution isotachophoresis method includes a trailing electrolyte solution adapted to migrate slower than the target cells and a leading electrolyte solution adapted to migrate faster than the target cells. A mixed-cell sample containing the target and non-target cells is slurried in the trailing electrolyte solution prior to cell extraction.