The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2020

Filed:

Oct. 24, 2014
Applicants:

The Foundation for the Promotion of Industrial Science, Tokyo, JP;

Neuroindx, Inc., Signal Hill, CA (US);

Centre National DE LA Recherche Scientifique, Paris, FR;

Inventors:

Hiroyuki Fujita, Tokyo, JP;

Stanislav L. Karsten, Signal Hill, CA (US);

Dominique Collard, Tokyo, JP;

Momoko Kumemura, Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); G01N 27/327 (2006.01); C12Q 1/68 (2018.01); G01N 33/487 (2006.01); C12Q 1/6844 (2018.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 27/4473 (2013.01); C12Q 1/6844 (2013.01); G01N 27/3275 (2013.01); G01N 27/3278 (2013.01); G01N 27/44791 (2013.01); G01N 33/48721 (2013.01); B01L 3/5027 (2013.01);
Abstract

An object is to make a bridge of DNA expanding between a pair of electrodes, and to characterize the bridge of DNA, to thereby detect DNA easily and surely without employing any marker or labeling substances, such as fluorescent reagents. A method of detecting DNA using a detection device with at least a couple of electrodes, the method comprising immobilizing a primer on the electrodes; making a bridge of the DNA expanded between the electrodes, by immersing the electrodes in a solution including circular templates of single stranded DNA, annealing the circular templates, and generating single stranded DNA product utilizing RCA (Rolling Circle Amplification), with impressing a designated voltage between the electrodes; and characterizing the bridge of DNA which includes multiple single stranded DNA molecules between the electrodes.


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