IDiyas

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10844407 B1

PDFFull Text
Date of Patent:
Nov. 24, 2020

Filed:

Mar. 03, 2017

Variant type tetraprenyl-β-curcumene cyclase and method for producing ambrein

Applicants:

Niigata University, Niigata, JP;

Adeka Corporation, Tokyo, JP;

Inventors:

Tsutomu Sato, Niigata, JP;

Kotone Okuno, Niigata, JP;

Toshihiko Takehana, Tokyo, JP;

Seiji Koike, Tokyo, JP;

Assignees:

NIIGATA UNIVERSITY, Niigata, JP;

ADEKA CORPORATION, Tokyo, JP;

Attorney:

Heslin Rothenberg Farley & Mesiti P.C.

Primary Examiner:

Ganapathirama Raghu

Int. Cl.
CPC ...
C12P 7/04 (2006.01); C12N 9/90 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12P 7/02 (2006.01); C12N 1/20 (2006.01); C12N 1/32 (2006.01); C12N 9/04 (2006.01); C12N 9/88 (2006.01);
U.S. Cl.
CPC ...
C12P 7/04 (2013.01); C12N 1/20 (2013.01); C12N 1/32 (2013.01); C12N 5/10 (2013.01); C12N 9/0006 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/09 (2013.01); C12P 7/02 (2013.01); C12Y 101/01034 (2013.01); C12Y 402/01137 (2015.07);
Abstract

An object of the present invention is to provide a method for preparing ambrein, which can easily obtain the ambrein. The object can be solved by a mutated tetraprenyl-β-curcumene cyclase wherein a fourth amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, (a) having a QXXXGX(W/F) motif, and a QXXXX(G/A)X(F/W/Y) motif on the N-terminal side, and a QXXXGX(F/W/Y) motif, and a QXXXGXW motif and a QXXXGX(F/W) motif on the C-terminal side, and not having a QXXXGXW motif at a position separated by 170 amino acid residues or more on the C-terminal side, with respect to the DXDD motif, (b) having 40% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 13, (c) exhibiting ambrein production activity using squalene as a substrate.

Find Patent Forward Citations

Loading…