The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2020

Filed:

Nov. 18, 2015
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Xiaowei Xu, Monmouth Junction, NJ (US);

Ruifeng Yang, Philadelphia, PA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 5/071 (2010.01); A61K 35/36 (2015.01); A61P 17/00 (2006.01); A61L 27/38 (2006.01); A61L 27/54 (2006.01); A61L 27/58 (2006.01); A61L 27/60 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0626 (2013.01); A61K 35/36 (2013.01); A61L 27/3813 (2013.01); A61L 27/3839 (2013.01); A61L 27/3886 (2013.01); A61L 27/3895 (2013.01); A61L 27/54 (2013.01); A61L 27/58 (2013.01); A61L 27/60 (2013.01); A61P 17/00 (2018.01); G01N 33/5023 (2013.01); A61L 2300/64 (2013.01); A61L 2430/34 (2013.01); C12N 2501/01 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/365 (2013.01); C12N 2501/60 (2013.01); C12N 2506/1307 (2013.01); C12N 2510/00 (2013.01);
Abstract

Compositions and methods for generating melanocytes through direct reprogramming are disclosed. Also disclosed are methods of use of such compositions for the treatment of vitiligo and other hypopigmentation disorders. In accordance with the present invention, a method for producing melanocytes suitable for use in human patients is provided. An exemplary method comprises providing cells capable of transdifferentiation into melanocytes, culturing said cells in a chemically defined culture medium, introducing at least two of microphthalmia-associated transcription factor (MITF), SRY-related HMG-box (SOX10) transcription factor and paired box-3 (PAX-3) transcription factor and paired box-3 (PAX-3) transcription factor, or nucleic acids encoding said transcription factors into said cells, wherein expression of said factors induces the cells to transdifferentiae into melanocytes expressing melanocyte markers TYR, DCT, S-100 and Melan-A.


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