The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 17, 2020
Filed:
Oct. 26, 2018
Flagship Biosciences, Inc., Westminster, CO (US);
Cris L. Luengo Hendriks, Broomfield, CO (US);
Flagship Biosciences, Inc., Westminster, CO (US);
Abstract
We present a method to detect dots in microscope images of tissue samples for the purpose of diagnosis or therapy selection. Dots in tissue arise trough CISH or FISH staining, but also through staining of small cellular compartments, or in other ways. The method is applicable to both brightfield and fluorescence modalities, as well as mass spectrometry imaging methodologies. The method is based on the use of first and second derivatives of the image along the image axes, computed using regularized derivative operators, i.e. Gaussian derivatives. The outputs of these operators are examined at appropriate distances from each location in the image. The found values must have specific signs. If the signs are all correct, these values are multiplied together to obtain a confidence measure for a dot being present at that location. If the signs do not match, no dot is present, and a zero is output.