Super-resolution fluorescent imaging probe

Abstract

[Problem] To provide a novel fluorescent probe for super-resolution imaging that uses fluorescent light emission characteristics that originate from an intermolecular nucleophilic addition-dissociation equilibrium reaction, and to provide a super-resolution fluorescent imaging method that uses the probe. [Solution] A fluorescent probe for super-resolution imaging that comprises a compound represented by formula (I) or a salt thereof [in the formula, X represents an oxygen atom, C(R)(R), or Si(R)(R) (wherein Rand Reach independently represent a hydrogen atom or an alkyl group), Rrepresents a hydrogen atom or an optionally substituted aryl (provided that, if Ris a phenyl group, the benzene ring of the phenyl group does not have a substituent at position 2 or position 6), Rand Reach independently represent 1-3 identical or differing substituents that are independently selected from the group that consists of hydrogen atoms, hydroxyl groups, halogen atoms, optionally substituted alkyl groups, optionally substituted sulfo groups, optionally substituted carboxyl groups, optionally substituted ester groups, optionally substituted amide groups, and optionally substituted azide groups, Rand Reach independently represent a hydrogen atom or an optionally substituted alkyl group or N(R)(R) forms an amide group or a carbamate group (provided that, if Ror Ris an alkyl group, each may form, together with R, a ring structure that contains the nitrogen atom that is bonded thereto), and Rand Reach independently represent a hydrogen atom or an optionally substituted alkyl group or N(R)(R) forms an amide group or a carbamate group (provided that, if Ror Ris an alkyl group, each may form, together with R, a ring structure that contains the nitrogen atom that is bonded thereto)], the fluorescent probe for super-resolution imaging being characterized in that the compound represented by formula (I) or the salt thereof undergoes a nucleophilic addition-dissociation equilibrium reaction with a nucleophilic compound that contains an —SH group.