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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10837035 B1

PDFFull Text
Expired
Date of Patent:
Nov. 17, 2020

Filed:

May. 31, 2020

Method for extracting citrulline from watermelon

Applicant:

Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences, Zhangzhou, CN;

Inventors:

Zhonggao Jiao, Zhengzhou, CN;

Jiechao Liu, Zhengzhou, CN;

Chunling Zhang, Zhengzhou, CN;

Qiang Zhang, Zhengzhou, CN;

Hui Liu, Zhengzhou, CN;

Zhenzhen Lv, Zhengzhou, CN;

Wenbo Yang, Zhengzhou, CN;

Dalei Chen, Zhengzhou, CN;

Assignee:

ZHENGZHOU FRUIT RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Zhengzhou, CN;

Attorney:

SZDC Law P.C.

Primary Examiner:

Robert J Yamasaki

Assistant Examiner:

Charles Zoltan Constantine

Int. Cl.
CPC ...
C12P 13/10 (2006.01); C07C 273/18 (2006.01); B01D 9/00 (2006.01); B01D 11/02 (2006.01); B01D 15/36 (2006.01);
U.S. Cl.
CPC ...
C12P 13/10 (2013.01); B01D 9/00 (2013.01); B01D 11/0265 (2013.01); B01D 11/0288 (2013.01); B01D 15/361 (2013.01); C07C 273/189 (2013.01);
Abstract

The present invention relates to the technical field of natural substance extraction. Disclosed is a method for extracting Citrulline from a watermelon. The method for extracting Citrulline from a watermelon in the present invention comprises Raw material pretreatment, ultrasound-enzymatic hydrolysis assisted solvent leaching, and purification steps. The purification step comprises microbial fermentation, ion-exchange resin purification, macroporous adsorption resin discoloration, and crystallization and recrystallization. The method for extracting Citrulline in the present invention is simple, requires a mild condition, and has a good extraction effect on Citrulline. The purity of Citrulline after purification is more than 90%. In addition, the Citrulline extracted by the method in the present invention meets the related health requirements and product quality standards, can be applied to food and health food industries as a raw material, and has natural security advantages.

Find Patent Forward Citations

Jiao, Zhonggao. (2020). Method for extracting citrulline from watermelon (U.S. Patent No. 10837035). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10837035

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