The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 17, 2020

Filed:

Apr. 03, 2019
Applicant:

C3j Therapeutics, Inc., Marina Del Rey, CA (US);

Inventors:

Kyle C. Cady, San Diego, CA (US);

E. Magda Barbu, San Diego, CA (US);

Christen G. DiPetrillo, San Diego, CA (US);

Assignee:

C3J Therapeutics, Inc., Marina Del Rey, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C12N 9/22 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C12N 9/22 (2013.01); C12P 19/34 (2013.01); C12N 2710/16121 (2013.01); C12N 2795/10221 (2013.01); C12N 2795/10222 (2013.01); C12N 2795/10321 (2013.01); C12N 2795/10322 (2013.01); C12N 2795/14121 (2013.01); C12N 2795/14122 (2013.01); C12Y 301/00 (2013.01);
Abstract

The present disclosure relates to a method of in vitro engineering of nucleic acids. This disclosure further relates to in vitro engineering of viral genomes and to the improvement of viral properties by in vitro genomic engineering of viral genomes. Specifically, the disclosure relates to in vitro viral genomic digestion using RNA-guided Cas9, the assembly of a recombinant genome by the insertion of a DNA or RNA fragment into the digested viral genome and transformation of a host cell with the recombinant genome. This method also related to in vitro engineering for error correction of nucleic acids.


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