The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 03, 2020
Filed:
Jun. 01, 2017
Bioright Worldwide Co., Ltd., Road Town, VG;
Yuk Sun Lui, Hong Kong, CN;
Shiu Ming Cheng, Hong Kong, CN;
Yuen Wing Chow, Hong Kong, CN;
Yau Lung Siu, Hong Kong, CN;
Jun Wang, Hong Kong, CN;
BioRight Worldwide Co., Ltd., Tortola, VG;
Abstract
This invention provides deacetoxycephalosporin C hydroxylase mutants, their encoding DNA sequences, the methods to utilize them and their application. The deacetoxycephalosporin C hydroxylase mutants are characterized by at least one amino acid mutation at residue position selected from Glycine at position 29, Alanine at position 40, Glycine at position 41, Arginine at position 182 and Threonine at position 272, based on the amino acid sequence shown in SEQ ID NO.:2 as reference sequence, and wherein the deacetoxycephalosporin C hydroxylase mutant has at least 10% increase in activity and at least 150% increase in thermostability compared to wild-type deacetoxycephalosporin C hydroxylase. The deacetoxycephalosporin C hydroxylase mutants in this invention have increased activity and thermostability, allowing them to be more commercially and industrially viable.