The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 03, 2020

Filed:

Jul. 10, 2019
Applicant:

Celsee Diagnostics, Inc., Plymouth, MI (US);

Inventors:

Kalyan Handique, Ann Arbor, MI (US);

Vishal Sharma, Plymouth, MI (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); G01N 33/574 (2006.01); C12Q 1/6816 (2018.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 15/14 (2006.01); C12Q 1/6886 (2018.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); C12M 47/04 (2013.01); C12Q 1/6816 (2013.01); G01N 15/1434 (2013.01); G01N 15/1484 (2013.01); G01N 33/543 (2013.01); G01N 33/569 (2013.01); G01N 33/574 (2013.01); B01L 3/502715 (2013.01); B01L 3/527 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0851 (2013.01); B01L 2400/0406 (2013.01); C12Q 1/6886 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/149 (2013.01);
Abstract

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.


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