The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 27, 2020
Filed:
Sep. 28, 2018
Ikeda Food Research Co., Ltd., Fukuyama-shi, Hiroshima-ken, JP;
Phc Corporation, Ehime, JP;
Hironori Omura, Hiroshima-ken, JP;
Hirokazu Sanada, Hiroshima-ken, JP;
Takako Yada, Hiroshima-ken, JP;
Ayaka Atsumi, Hiroshima-ken, JP;
Tetsunari Morita, Hiroshima-ken, JP;
Emi Ishimaru, Hiroshima-ken, JP;
Ikeda Food Research Co., Ltd., Fukuyama-shi, Hiroshima-ken, JP;
PHC Corporation, Ehime, JP;
Abstract
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.