The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 27, 2020

Filed:

Jul. 17, 2018
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Karl Martin Hjort, Lund, SE;

Sergio Leal-Ortiz, Hayward, CA (US);

Yuhong Cao, Palo Alto, CA (US);

Chris Rehse, Palo Alto, CA (US);

Andy Kah Ping Tay, Stanford, CA (US);

Nicholas A. Melosh, Menlo Park, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/87 (2006.01); C12M 1/42 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12N 5/00 (2006.01); B82Y 40/00 (2011.01); B82Y 5/00 (2011.01);
U.S. Cl.
CPC ...
C12N 15/87 (2013.01); C12M 23/02 (2013.01); C12M 23/42 (2013.01); C12M 35/00 (2013.01); C12M 35/02 (2013.01); C12M 35/04 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12M 23/16 (2013.01); C12N 5/0068 (2013.01); C12N 5/0075 (2013.01); C12N 2521/00 (2013.01); C12N 2535/00 (2013.01);
Abstract

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.


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