The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 27, 2020
Filed:
Feb. 08, 2018
Applicant:
Amicus Therapeutics, Inc., Cranbury, PA (US);
Inventor:
Hung Do, New Hope, PA (US);
Assignee:
Amicus Therapeutics, Inc., Cranbury, NJ (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 47/64 (2017.01); C12N 9/16 (2006.01); C12N 9/20 (2006.01); C12N 9/24 (2006.01); C12N 9/26 (2006.01); C12N 9/42 (2006.01); C12N 9/40 (2006.01); C07K 14/65 (2006.01); A61K 38/30 (2006.01); A61K 38/47 (2006.01); C12N 9/96 (2006.01); A61K 47/60 (2017.01);
U.S. Cl.
CPC ...
A61K 47/64 (2017.08); A61K 38/30 (2013.01); A61K 38/47 (2013.01); A61K 47/60 (2017.08); C07K 14/65 (2013.01); C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12N 9/2402 (2013.01); C12N 9/2408 (2013.01); C12N 9/2434 (2013.01); C12N 9/2465 (2013.01); C12N 9/2488 (2013.01); C12N 9/96 (2013.01); C07K 2319/00 (2013.01); C12Y 302/0102 (2013.01);
Abstract
Disclosed herein are methods of chemical conjugation comprising contacting a lysosomal enzyme with a first crosslinking agent to introduce aldehyde groups; contacting a lysosomal targeting peptide with a second crosslinking agent to introduce a hydrazide group at the N-terminal residue; contacting the lysosomal enzyme with aldehyde groups of step a. with the lysosomal targeting peptide with a hydrazide group at the N-terminal residue of step b; and forming a lysosomal enzyme-lysosomal targeting peptide conjugate.