The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 20, 2020

Filed:

Nov. 14, 2018
Applicant:

Advanced Liquid Logic, Inc., San Diego, CA (US);

Inventors:

Vamsee K. Pamula, Cary, NC (US);

Ramakrishna Sista, Cary, NC (US);

Vijay Srinivasan, Cary, NC (US);

Michael G. Pollack, Durham, NC (US);

Allen E. Eckhardt, Durham, NC (US);

Assignee:

ADVANCED LIQUID LOGIC, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/40 (2006.01); G01N 1/00 (2006.01); G01N 1/10 (2006.01); G01N 1/22 (2006.01); G01N 1/18 (2006.01); G01N 35/00 (2006.01); G01N 1/38 (2006.01); B01L 3/00 (2006.01); G01N 33/543 (2006.01); B01F 11/00 (2006.01); B01F 13/00 (2006.01); B01F 13/08 (2006.01); B01L 7/00 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54326 (2013.01); B01F 11/0071 (2013.01); B01F 13/0071 (2013.01); B01F 13/0076 (2013.01); B01F 13/0809 (2013.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); B01L 3/502792 (2013.01); G01N 1/38 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/089 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0442 (2013.01); G01N 2001/386 (2013.01); G01N 2035/1046 (2013.01); Y10T 436/11 (2015.01); Y10T 436/25 (2015.01); Y10T 436/2575 (2015.01); Y10T 436/25625 (2015.01);
Abstract

Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.


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