The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 20, 2020

Filed:

Oct. 14, 2016
Applicant:

Plasma Technologies, Llc, Kiawah Island, SC (US);

Inventors:

Eugene Zurlo, Charleston, SC (US);

Dennis Curtin, Plattsburgh, NY (US);

Assignee:

Plasma Technologies, LLC, Charleston, SC (US);

Attorney:
Int. Cl.
CPC ...
G01N 33/49 (2006.01); C07K 1/18 (2006.01); C07K 1/22 (2006.01); C07K 1/32 (2006.01); C07K 14/81 (2006.01); B01D 15/12 (2006.01); B01D 15/38 (2006.01); G01N 30/02 (2006.01); G01N 30/88 (2006.01);
U.S. Cl.
CPC ...
G01N 33/491 (2013.01); B01D 15/12 (2013.01); B01D 15/3804 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/32 (2013.01); C07K 14/81 (2013.01); C07K 14/8125 (2013.01); G01N 30/02 (2013.01); G01N 2030/8822 (2013.01);
Abstract

A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields.


Find Patent Forward Citations

Loading…