The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 20, 2020

Filed:

Dec. 16, 2015
Applicant:

Corning Incorporated, Corning, NY (US);

Inventors:

Ye Fang, Painted Post, NY (US);

Ann MeeJin Ferrie, Painted Post, NY (US);

Vasiliy Nikolaevich Goral, Painted Post, NY (US);

David Henry, Fontaine le Port, FR;

Martial Hervy, Veneux les sablons, FR;

Corinne Walerack, Veneux les sablons, FR;

Yue Zhou, Horseheads, NY (US);

Assignee:

Corning Incorporated, Corning, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C08J 3/24 (2006.01); C09D 167/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0075 (2013.01); C08J 3/24 (2013.01); C09D 167/00 (2013.01); C12N 5/0068 (2013.01); C12N 2533/32 (2013.01); C12N 2533/40 (2013.01); C12N 2533/50 (2013.01); C12N 2533/74 (2013.01); C12N 2537/10 (2013.01);
Abstract

A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.


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