The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 13, 2020
Filed:
Jul. 15, 2019
Use of ion concentrations to increase the packaging efficiency of recombinant adeno-associated virus
Applicant:
Vigene Biosciences Inc., Rockville, MD (US);
Inventor:
Qizhao Wang, Rockville, MD (US);
Assignee:
Vigene Biosciences, Inc., Rockville, MD (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/86 (2006.01); C12N 7/00 (2006.01); C12N 5/16 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C12N 5/16 (2013.01); C12N 7/00 (2013.01); C12N 2500/14 (2013.01); C12N 2500/16 (2013.01); C12N 2710/10041 (2013.01);
Abstract
The present invention is directed to methods for increasing the efficiencies with which recombinant adeno-associated virus (rAAV) are packaged, so as to increase their production titers. More specifically, the invention relates to a method for increasing the production titer of rAAV by transfected cells by increasing the ionic strength of the cell culture media through the administration of additional ions.