The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 13, 2020

Filed:

Oct. 13, 2017
Applicant:

Hitgen Ltd, Chengdu, CN;

Inventors:

Jin Li, Chengdu, CN;

Jinqiao Wan, Chengdu, CN;

Guansai Liu, Chengdu, CN;

Dengfeng Dou, Chengdu, CN;

Assignee:

Hitgen INC., Chengdu, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C40B 50/00 (2006.01); C40B 70/00 (2006.01); C40B 40/06 (2006.01); C40B 50/04 (2006.01); C40B 50/16 (2006.01); C40B 20/04 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1068 (2013.01); C12P 19/34 (2013.01); C40B 20/04 (2013.01); C40B 40/06 (2013.01); C40B 50/04 (2013.01); C40B 50/16 (2013.01); C40B 70/00 (2013.01);
Abstract

The present invention provides a method of solid-phase synthesis of DNA-encoded compound library. The method includes following steps: a) reacting solid carrier G-1 with linker molecule L-1 to prepare L-G-1; b) reacting DNA with linker molecule L-0 to prepare L-2; c) reacting L-G-1 with L-2 to prepare L-G-2; d) removing protection group of the L-G-2 and obtaining L-G-2-1; e) reacting the L-G-2-1 with synthetic building block and performing DNA encoding; and f) removing the solid carrier and obtaining the DNA-encoded compound library. Compared with the prior art, the present invention can complete post-treatment purification of the reaction only by filtration and irrigation processes for several times. The present invention is simple to operate, can shorten the production cycle of DNA encoded compound library with more than 50%, significantly increases the production efficiency and the unicity as well as the purity of the final products.


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