The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 06, 2020
Filed:
Dec. 07, 2015
Howard Hughes Medical Institute, Ashburn, VA (US);
Robert Eric Betzig, Ashburn, VA (US);
Wesley Legant, Fairfax, VA (US);
Howard Hughes Medical Institute, Ashburn, VA (US);
Abstract
A sample is bathed in a solution that includes labels having a binding affinity for a structure on the sample and that emit light in response to excitation light. A sheet of excitation light having a FWHM thickness is provided to the sample. Light emitted from labels in response to the excitation light is imaged onto a detector, where the light is imaged with a detection objective having a depth of focus comparable to or greater than the FWHM thickness. The bathing of the sample and the imaging of the light emitted from the bound labels is controlled, such that the imaged light from different individual labels bound to the structure is resolved on the detector. The providing, imaging, and controlling are repeated to image, at different times, light from labels bound to the structure at different locations on or within the sample.