The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 06, 2020

Filed:

Mar. 07, 2018
Applicant:

Oilcrops Research Institute of Chinese Acadamy of Agriculture Sciences, Hubei, CN;

Inventors:

Zhaowei Zhang, Hubei, CN;

Peiwu Li, Hubei, CN;

Qi Zhang, Hubei, CN;

Xiaofeng Hu, Hubei, CN;

Wen Zhang, Hubei, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/577 (2006.01); B01J 20/286 (2006.01); B01D 15/38 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/577 (2013.01); B01D 15/3804 (2013.01); B01J 20/286 (2013.01); G01N 33/543 (2013.01); G01N 33/54366 (2013.01); G01N 33/54393 (2013.01); G01N 2333/37 (2013.01); G01N 2333/38 (2013.01); G01N 2333/385 (2013.01);
Abstract

An immunoadsorbent for purifying fumonisin B, aflatoxin B, ochratoxin A, zearalenone and sterigmatocystin; a complex affinity column and its preparation method; and a method for detecting the mycotoxins using the same are provided. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin Bmonoclonal antibody, an anti-aflatoxin Bmonoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin Bmonoclonal antibody is secreted by a hybridoma cell strain Fm7A11. The complex affinity column can be used for purification and detection of a fumonisin Bsample, an aflatoxin Bsample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.


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