The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 29, 2020

Filed:

Sep. 28, 2017
Applicant:

General Automation Lab Technologies, Inc., San Francisco, CA (US);

Inventors:

Peter Christey, San Francisco, CA (US);

Alexander Hallock, Redwood City, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6888 (2018.01); C07H 21/00 (2006.01); G01N 27/00 (2006.01); G01N 27/447 (2006.01); G01N 33/569 (2006.01); C12Q 1/06 (2006.01); C12M 1/04 (2006.01); C12M 1/32 (2006.01); C12Q 1/6853 (2018.01); B01L 3/00 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/689 (2018.01); B01L 3/02 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44791 (2013.01); B01L 3/0244 (2013.01); B01L 3/5085 (2013.01); C12M 23/12 (2013.01); C12M 23/24 (2013.01); C12Q 1/06 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6853 (2013.01); G01N 33/56911 (2013.01); B01L 2200/025 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2200/141 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/165 (2013.01);
Abstract

A method is provided for analyzing a sample including a population of biological entities using at least one microfabricated device. A plurality of the microwells on the microfabricated device are each uniquely indexed, and loaded with a sample such that at least some microwells each include more than one cell of a biological entity. The microfabricated device was incubated at predetermined conditions, and a selected genetic material of the cells of the biological entities obtained from the incubation is amplified to obtaining amplicons. An aggregate of the amplicons are sequenced obtain sequencing data, based on which and the indexing of the microwells, an identification of the biological entities present in each of the plurality of microwells is obtained. Such identification can then be used to determine a relationship between different types of biological entities in the sample.


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