The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 29, 2020
Filed:
Sep. 20, 2018
Applicant:
Life Technologies As, Carlsbad, CA (US);
Inventors:
Darren Ellis, Lommedalen, NO;
Hannah Lindstroem, Oslo, NO;
Assignee:
LIFE TECHNOLOGIES AS, Oslo, NO;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); C12N 15/1003 (2013.01); C12Q 2527/125 (2013.01);
Abstract
The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.