The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 29, 2020

Filed:

Feb. 23, 2017
Applicant:

Dojindo Laboratories, Kamimashiki-gun, Kumamoto, JP;

Inventors:

Hidefumi Iwashita, Kumamoto, JP;

Ryo Sakamoto, Kumamoto, JP;

Munetaka Ishiyama, Kumamoto, JP;

Assignee:

DOJINDO LABORATORIES, Kamimashiki-Gun, Kumamoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07F 9/59 (2006.01); G01N 33/52 (2006.01); C07F 9/6558 (2006.01); G01N 21/64 (2006.01); G01N 21/77 (2006.01); G01N 33/58 (2006.01); C09K 11/06 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C07F 9/65583 (2013.01); C09K 11/06 (2013.01); G01N 21/64 (2013.01); G01N 21/77 (2013.01); G01N 33/5079 (2013.01); G01N 33/52 (2013.01); G01N 33/582 (2013.01); G01N 2800/2835 (2013.01);
Abstract

Disclosed is a pH-responsive fluorescent compound, represented by the general formula, which is a novel pH-responsive fluorescent compound capable of being specifically localized in mitochondria within cells, which exhibits strong fluorescence under weakly acidic pH environments in lysosomes, and which is not readily subject to interference from autofluorescence and background fluorescence due to other fluorescent substances within cells. Also disclosed are a composition for detecting mitophagy using the pH-responsive fluorescent compound, and a method for detecting mitophagy within cells. In the general formula, L represents a linker, X represents a pharmaceutically acceptable anion, and Y represents a reactive group that may react with a functional group on a mitochondrial protein to form a covalent bond.


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