The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 2020

Filed:

Nov. 29, 2017
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Steven Lytle, Kirkland, WA (US);

Paul Boeschoten, Redmond, WA (US);

Andrew Gunderson, Bothell, WA (US);

Larry Rystrom, Mill Creek, WA (US);

Chris Gnehm, Bothell, WA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G02B 21/00 (2006.01); G01N 21/27 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G02B 21/0076 (2013.01); G01N 21/274 (2013.01); G01N 21/6458 (2013.01); G02B 21/0032 (2013.01); G02B 21/0036 (2013.01); G02B 21/0044 (2013.01);
Abstract

A method for calibrating an imaging system can include at least the following method acts: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using a known spacing of pinholes in the pinhole mask to obtain filtered data that corresponds to the known spacing, using a threshold to identify regions of the filtered data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit data for the filtered data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.


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