Method and a system for determinations of cell suspensions

Abstract

The invention relates to a method of determining a concentration of a substance in a cell suspension, said method comprising the following steps: —determining the concentration of the substance by: —using the results of absorption measurements performed at n local sample volumes contained at different average chamber heights of a chamber arrangement comprising the cell suspension and local substance concentration in said respective local sample volumes determined based on said respective absorption measurements; —using a substance concentration model comprising local substance concentration as a function of chamber height, and—determining the substance concentration as the infinite chamber height substance concentration using said substance concentration model and the determined local substance concentrations, wherein n is at least 2, such as at least 3, such as at least 4, wherein, optionally, the cell suspension is whole blood and the substance is Hb and wherein the method further comprises determining cRBC based on the determined Hb concentration and a determined Mean Corpuscular Hemoglobin (MCH). The invention also relates to a system for executing the method.