The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 15, 2020
Filed:
Sep. 01, 2016
Applicant:
The Regents of the University of California, Oakland, CA (US);
Inventors:
Daniel A. Portnoy, Albany, CA (US);
Jonathan L. Portman, Berkeley, CA (US);
Assignee:
The Regents of the University of California, Oakland, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 39/02 (2006.01); C12Q 1/689 (2018.01); C07K 14/195 (2006.01); A61K 35/74 (2015.01); C12N 15/74 (2006.01); C12N 1/20 (2006.01); A61P 31/04 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/689 (2013.01); A61K 35/74 (2013.01); A61K 39/0208 (2013.01); A61P 31/04 (2018.01); C07K 14/195 (2013.01); C12N 1/20 (2013.01); C12N 15/74 (2013.01); A61K 2039/521 (2013.01); C12Q 2600/158 (2013.01);
Abstract
Methods of assessing expression of a PrfA mediated expression construct in a Listerial host cell are provided. Aspects of the methods include maintaining a Listerial host cell that includes a PrfA mediated expression construct in a defined medium containing a reducing agent and evaluating expression of a product from the expression construct to assess expression of the PrfA mediated expression construct. Also provided are methods of producing and using a Listerial vaccine, as well as defined media for use in such methods.