The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 15, 2020
Filed:
Jun. 16, 2016
East China University of Science and Technology, Shanghai, CN;
Fengqing Wang, Shanghai, CN;
Liqin Xu, Shanghai, CN;
Yongjun Liu, Shanghai, CN;
Haohao Liu, Shanghai, CN;
Liangbin Xiong, Shanghai, CN;
Dongzhi Wei, Shanghai, CN;
East China University Of Science And Technology, Shanghai, CN;
Abstract
The present invention relates to a genetically-engineeredstrain and a use thereof in the preparation of steroidal compounds. The genetically-engineeredstrain is awhich lacks of acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33, wherein acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33 respectively encode proteins as follows: having amino acid sequences according to SEQ ID NOs 4, 6 and 8; derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by preceding protein and having the same function as that of the preceding protein. The present invention constructs a genetically-engineeredstrain and applies it in preparing steroidal compounds, thereby enriching the types of valuable intermediates, improving the production efficiency and product quality of steroid drugs, reducing energy consumption in the steroid drugs production, simplifying production steps, and reducing production costs.