The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 2020

Filed:

Mar. 28, 2017
Applicant:

Biosyntagma, Llc, Scottsdale, AZ (US);

Inventors:

David Wayne Richardson, Chandler, AZ (US);

Dmitry Derkach, Gibert, AZ (US);

Colleen Ziegler, Tempe, AZ (US);

Steve Pemberton, Bedford, NH (US);

Assignee:

BIOSYNTAGMA, LLC, Scottsdale, AZ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); G01N 1/28 (2006.01); G01N 1/44 (2006.01); C12M 1/00 (2006.01); C12M 1/14 (2006.01); B01L 3/00 (2006.01); G16B 50/00 (2019.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12M 47/04 (2013.01); B01L 3/5027 (2013.01); B01L 3/502715 (2013.01); C12M 1/00 (2013.01); C12M 1/14 (2013.01); G01N 1/286 (2013.01); G01N 15/1463 (2013.01); G16B 50/00 (2019.02); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); G01N 2001/2886 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1497 (2013.01);
Abstract

A method for dissecting and collecting one or more cells from a tissue sample fixed to an inner surface of a microfluidic device is described. The tissue sample is in fluid communication with a channel having an inlet end and an outlet end defined by the microfluidic device. The method comprises flowing a first fluid through the channel with a fluid flow from the inlet end to the outlet end; powering a laser to direct laser energy into the channel to impinge upon the first fluid proximate a first region of the tissue sample and cause fluid cavitation to thereby ablate a first set of one or more cells from the tissue sample; and collecting the first set of one or more cells within a first sample container coupled to the outlet end.


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