The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 08, 2020
Filed:
Jan. 11, 2019
Applicant:
Biomadison, Inc., Del Mar, CA (US);
Inventors:
Ward C. Tucker, Monona, WI (US);
Timothy M. Piazza, McFarland, WI (US);
Assignee:
BioMadison, Inc., Del Mar, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); C12Q 1/37 (2006.01); G01N 21/64 (2006.01); C12Q 1/6897 (2018.01); A47C 15/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56911 (2013.01); A47C 15/006 (2013.01); C12Q 1/37 (2013.01); C12Q 1/6897 (2013.01); G01N 21/6408 (2013.01); G01N 21/6428 (2013.01); G01N 2333/33 (2013.01);
Abstract
Methods for a cell-based assay for Botulinum neurotoxin are provided in which a transfected cell that produces a reporting peptide is contacted with a Botulinum neurotoxin in media having a sub-physiological osmolarity and a temperature that is above physiological temperature. This combination provides an unexpected synergistic effect in reducing the ECof the cell-based assay relative to an analogous cell-based assay performed at physiological osmolarity and temperature.