The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 08, 2020

Filed:

Nov. 30, 2012
Applicants:

Enchi Corporation, Wellesley Hills, MA (US);

Dartmouth College, Hanover, NH (US);

Ut-battelle, Llc, Oak Ridge, TN (US);

Inventors:

Jonathan Lo, Hanover, NH (US);

Adam M. Guss, Knoxville, TN (US);

Johannes P. Van Dijken, Scheidam, NL;

Arthur J. Shaw, IV, Grantham, NH (US);

Daniel G. Olson, Norwich, VT (US);

Christopher D. Herring, Lebanon, NH (US);

D. Aaron Argyros, White River Junction, VT (US);

Nicky Caiazza, Rancho Santa Fe, CA (US);

Assignees:

Enchi Corporation, Wellesley Hills, MA (US);

Dartmouth College, Hanover, NH (US);

UT-Battelle, LLC, Oak Ridge, TN (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/21 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 15/53 (2006.01); C12P 7/06 (2006.01); C12N 15/74 (2006.01);
U.S. Cl.
CPC ...
C12P 7/06 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 15/74 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/01002 (2013.01); Y02E 50/17 (2013.01);
Abstract

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.


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