The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 08, 2020
Filed:
Apr. 20, 2017
Applicant:
Agilent Technologies, Inc., Santa Clara, CA (US);
Inventors:
Douglas J Dellinger, Boulder, CO (US);
Daniel E Ryan, San Francisco, CA (US);
Subhadeep Roy, Lafayette, CO (US);
Jeffrey R Sampson, San Jose, CA (US);
Assignee:
AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 15/11 (2013.01); C12N 15/111 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2310/312 (2013.01); C12N 2310/315 (2013.01); C12N 2310/3125 (2013.01); C12N 2310/321 (2013.01); C12N 2310/346 (2013.01); C12N 2320/10 (2013.01); C12N 2320/50 (2013.01);
Abstract
The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.