The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 01, 2020

Filed:

Dec. 16, 2015
Applicant:

Shire Human Genetic Therapies, Inc., Lexington, MA (US);

Inventors:

Peter Bernhardt, Lexington, MA (US);

Chen-Chung Willy Yen, Lexington, MA (US);

Vijay Chhajlani, Arlington, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/40 (2006.01); C12Q 1/34 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/40 (2013.01); C12Q 1/34 (2013.01); C12Y 302/01045 (2013.01); G01N 2333/924 (2013.01); G01N 2500/20 (2013.01);
Abstract

The present invention provides, among other things, methods and compositions for determining enzyme kinetic parameters (e.g., Vmax, Km, and specific activity, etc.) indicative of clinically relevant properties of glucocerebrosidase using a physiologically relevant substrate, in particular, a substrate that is representative of substrates that typically accumulate in patients suffering from Gaucher disease such as glucosylceramide. Thus, the present invention is particularly useful to measure a kinetic parameter relating to the activity of glucocerebrosidase in a drug substance, drug product, and stability sample for enzyme replacement therapy.


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