The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 01, 2020

Filed:

Aug. 31, 2009
Applicants:

Kanako Usui, Otsu, JP;

Takashi Uemori, Otsu, JP;

Hiroyuki Mukai, Otsu, JP;

Ikunoshin Kato, Otsu, JP;

Inventors:

Kanako Usui, Otsu, JP;

Takashi Uemori, Otsu, JP;

Hiroyuki Mukai, Otsu, JP;

Ikunoshin Kato, Otsu, JP;

Assignee:

TAKARA BIO INC., Kusatsu-Shi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1096 (2013.01); C12Q 1/6848 (2013.01);
Abstract

A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.


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