The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 25, 2020
Filed:
Oct. 28, 2016
Applicant:
Leadway (Hk) Limited, Hong Kong, CN;
Inventors:
Assignee:
LEADWAY (HK) LIMITED, Hong Kong, CN;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/54 (2006.01); C12N 5/16 (2006.01); C12Q 1/00 (2006.01); C12N 5/10 (2006.01); C12N 9/04 (2006.01); C12Q 1/32 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/54 (2013.01); C12N 5/16 (2013.01); C12Q 1/006 (2013.01); C12Q 1/32 (2013.01); C12Y 101/05002 (2013.01);
Abstract
The present invention provides a soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-sGDH) mutant, wherein the amino acid positions thereof correspond to a wild-type PQQ-sGDH sequence ofas shown in SEQ ID NO 1 comprising one of the following group of mutations: A194F, a combined mutation based on A194F, a combined mutation based on Q192A or a combined mutation based on Q192S. Said PQQ-sGDH mutant has good glucose substrate specificity and significantly reduced cross-reactivity to maltose and the like, and is suitable for detecting glucose in a sample such as blood.