The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 25, 2020

Filed:

May. 04, 2016
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Christina D. Smolke, Menlo Park, CA (US);

Catherine Thodey, Mountain View, CA (US);

Isis Trenchard, Redwood City, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12P 17/12 (2006.01); C12P 13/22 (2006.01); C12N 15/81 (2006.01); C12N 15/79 (2006.01); C07D 217/22 (2006.01); C07D 217/18 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 15/79 (2013.01); C12N 15/81 (2013.01); C12P 13/22 (2013.01); C12P 17/12 (2013.01); C12Y 114/16002 (2013.01); C12Y 401/01028 (2013.01); C07D 217/18 (2013.01); C07D 217/22 (2013.01);
Abstract

Methods and engineered yeast cells for generating a benzylisoquinoline alkaloid product are provided herein. A method comprises providing engineered yeast cells and a feedstock to a reactor. In the reactor, the engineered yeast cells are subjected to fermentation by incubating the engineered yeast cells for a time period to produce a solution comprising the BIA product and cellular material. The solution comprises not more than one class of molecule selected from the group of protoberberine, morphinan, isopavine, aporphine, and benzylisoquinoline. Additionally, at least one separation unit is used to separate the BIA product from the cellular material to provide the product stream comprising the BIA product.


Find Patent Forward Citations

Loading…