The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 25, 2020

Filed:

May. 29, 2015
Applicants:

Corning Incorporated, Corning, NY (US);

Public University Corporation Yokohama City University, Yokohama-shi, JP;

Inventors:

Yoko Ejiri, Tsukuba, JP;

Hideki Taniguchi, Yokohama, JP;

Takanori Takebe, Yokohama, JP;

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/077 (2010.01); C12N 5/071 (2010.01); C12N 5/10 (2006.01); C12N 1/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); C12N 1/00 (2013.01); C12N 5/069 (2013.01); C12N 5/0652 (2013.01); C12N 5/0671 (2013.01); C12N 5/0697 (2013.01); C12N 5/10 (2013.01); C12N 2502/1352 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01); C12N 2531/00 (2013.01); C12N 2533/30 (2013.01);
Abstract

The present invention provides a culture method for culturing, in recesses (), a population including two or more cells including a cell derived from a stem cell and a mesenchymal cell. The cell derived from a stem cell is a cell obtained by differentiating a stem cell in vitro. The cell is a cell of one or more types selected from the group consisting of an endodermal cell, an ectodermal cell, and a mesodermal cell. The population is cultured in the recesses () together with a vascular cell or a secretor factor. Each recess () includes a space in which cells are movable. When a volume of the space is represented by V mmand the number of mesenchymal cells seeded in the space is represented by N, V is 400 or less and N/V is in a range from 35 to 3000.


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