The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 18, 2020
Filed:
Jun. 11, 2018
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Tobias Killian, Penzberg, DE;
Markus Neubauer, Penzberg, DE;
Assignee:
Hoffmann-La Roche Inc., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 5/00 (2006.01); C12P 21/02 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C07K 16/44 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C12N 5/0018 (2013.01); C12N 5/0602 (2013.01); C07K 16/44 (2013.01); C12N 2500/30 (2013.01); C12N 2501/11 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/25 (2013.01); C12N 2510/00 (2013.01);
Abstract
Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.