The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 18, 2020
Filed:
Aug. 28, 2015
Applicant:
Ucl Business Plc, London, GB;
Inventors:
Assignee:
UCL BUSINESS LTD, London, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/70 (2006.01); C12N 15/86 (2006.01); C07K 14/705 (2006.01); C12N 15/64 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C07K 14/70517 (2013.01); C12N 15/64 (2013.01); C07K 2319/03 (2013.01); C07K 2319/21 (2013.01); C07K 2319/22 (2013.01); C07K 2319/23 (2013.01); C07K 2319/50 (2013.01); C12N 2740/10041 (2013.01); C12N 2740/10051 (2013.01); C12N 2740/10052 (2013.01); C12N 2740/15043 (2013.01); C12N 2740/15051 (2013.01);
Abstract
The present invention relates to a producer cell which expresses a tagging protein at the cell surface, such that retroviral vectors produced by the cell are tagged with the tagging protein, wherein the tagging protein comprises: i) a binding domain which binds to a capture moiety ii) a spacer; and iii) a membrane targeting domain such that, when incorporated a retroviral vector, the tagging protein facilitates purification of the retroviral vector from cellular supernatant via binding of the tagging protein to the capture moiety. The present invention also relates to a retroviral vector comprising such a producer cell-derived tagging protein.