The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2020

Filed:

Aug. 04, 2014
Applicants:

The University of Tokyo, Tokyo, JP;

Peptidream Inc., Kanagawa, JP;

Inventors:

Hiroshi Murakami, Tokyo, JP;

Takashi Kawakami, Tokyo, JP;

Patrick Reid, Tokyo, JP;

Toru Sasaki, Tokyo, JP;

Assignees:

The University of Tokyo, Tokyo, JP;

PeptiDream Inc., Kanagawa, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C07K 7/06 (2006.01); C12N 15/67 (2006.01); C12P 21/02 (2006.01); G01N 33/68 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1062 (2013.01); C07K 7/06 (2013.01); C12N 15/67 (2013.01); C12P 21/02 (2013.01); G01N 33/5308 (2013.01); G01N 33/6845 (2013.01); Y02P 20/55 (2015.11);
Abstract

An object of the present invention is to provide a method of producing a peptide containing a charged non-proteinogenic amino acid in a cell-free translation system, and the like. The present invention provides a method of producing a peptide containing a charged non-proteinogenic amino acid. It includes a step of expressing a peptide in a cell-free translation system including (i) at least one tRNA to which a non-proteinogenic amino acid having a protecting-group-introduced charged group has been bound and (ii) a nucleic acid that encodes the peptide and contains at least one codon corresponding to an anticodon of the tRNA; and a step of removing the protecting group of the non-proteinogenic amino acid residue contained in the peptide.


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