The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 2020

Filed:

Aug. 20, 2018
Applicant:

Fundamental Solutions Corporation, Easton, PA (US);

Inventors:

M. Russell Williams, Easton, PA (US);

Joel Lwande, Athens, OH (US);

Shengwen Liang, Athens, OH (US);

Kyle McQuaid, Athens, OH (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); G01N 33/542 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
G01N 33/582 (2013.01); C12N 9/0069 (2013.01); C12Y 113/12005 (2013.01); G01N 33/542 (2013.01); C07K 2319/60 (2013.01); G01N 2333/90241 (2013.01);
Abstract

A bioassay for the detection of multiple target analytes that includes living immunocytes of a plurality of predetermined types; a signal-generating reporter within each immunocyte that is responsive to predetermined changes occurring within the immunocyte and that includes a bioluminescent protein linked to a fluorescent protein; a signal transduction pathway associated with each signal-generating reporter that is operative to induce the predetermined changes within each immunocyte; a detector element associated with the signal transduction pathway that is operative to trigger the signal transduction pathway; an analyte binding element associated with the detector element that is specific to both the detector element and a target analyte; and wherein upon the binding of an analyte binding element to which a target analyte is also bound to the detector element, the detector element triggers the signal transduction pathway causing the predetermined changes to occur within the living biological cell, thereby causing the signal-generating reporter to generate a detectable signal.


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