The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 2020

Filed:

Dec. 18, 2008
Applicants:

Takanori Kubo, Hiroshima, JP;

Hideki Ohba, Tosu, JP;

Kazuo Sakurai, Kitakyushu, JP;

Jusaku Minari, Kitakyushu, JP;

Atsushi Uno, Koganei, JP;

Inventors:

Takanori Kubo, Hiroshima, JP;

Hideki Ohba, Tosu, JP;

Kazuo Sakurai, Kitakyushu, JP;

Jusaku Minari, Kitakyushu, JP;

Atsushi Uno, Koganei, JP;

Assignee:

NAPA JENOMICS CO., LTD., Koganei-Shi, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 15/11 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C12N 2310/14 (2013.01); C12N 2310/3519 (2013.01); C12N 2320/32 (2013.01); C12N 2320/51 (2013.01);
Abstract

An object of the present invention is to provide novel double-stranded RNA having an RNA interference effect, in which the cellular uptake and the resistance to enzymatic degradation are improved, without reducing the RNA interference effect. The cellular uptake and the resistance to enzymatic degradation of a complex of a polysaccharide having a β-1,3-glucan skeleton and double-stranded RNA can be significantly improved while maintaining the RNA interference effect, by fulfilling the following conditions (i) to (iii): (i) the double-stranded RNA has a sense strand consisting of a base sequence complementary to a target sequence in a target gene and an antisense strand containing a base sequence complementary to the sense strand, and the double-stranded RNA can inhibit expression of the target gene; (ii) the double-stranded RNA has a single-stranded polydeoxyadenine bound directly or via a linker to the end of at least one of the sense strand and the antisense strand; and (iii) the polysaccharide and the single-stranded polydeoxyadenine form a complex.


Find Patent Forward Citations

Loading…